SELECTED Publications

2024

• Mueller L M, Isaacson A, Wilson H, Salowka A, Gong M, Raile K, Spagnoli FM. Heterozygous missense variant in GLI2 impairs human endocrine pancreas development. Nat Commun. 2024 15, 2483. https://doi.org/10.1038/s41467-024-46740-8 [PubMed]

• Darrigrand JF, Salowka A, Torres-Cano A, Tapia-Rojo R, Zhu T, Garcia-Manyes S., Spagnoli FM. Acinar-ductal cell rearrangement drives branching morphogenesis of the murine pancreas in an IGF/PI3K-dependent manner. Dev Cell 2024 59(3):326-338.e5. doi: 10.1016/j.devcel.2023.12.011. Epub 2024 Jan 17. [PubMed]

2023

• Zhu H, Wang G, Nguyen-Ngoc KV, Kim D, Miller M, Goss G, Kovsky J, Harrington AR, Saunders DC, Hopkirk AL, Melton R, Powers AC, Preissl S, Spagnoli FM, Gaulton KJ, Sander M. Understanding cell fate acquisition in stem-cell-derived pancreatic islets using single-cell multiome-inferred regulomes. Dev Cell. 2023 May 8;58(9):727-743.e11. doi: 10.1016/j.devcel.2023.03.011. [PubMed]

• Ortega, S.G., Melati, A., Menne, V., Salowka, A., Segoviano, M.V., Spagnoli, F.M. (2023). Pancreatic Cell Fate Specification: Insights Into Developmental Mechanisms and Their Application for Lineage Reprogramming. In: Piemonti, L., Odorico, J., Kieffer, T.J., Sordi, V., de Koning, E. (eds) Pluripotent Stem Cell Therapy for Diabetes. Springer, Cham. https://doi.org/10.1007/978-3-031-41943-0_3 [PubMed]

• Goldrick C, Guri I, Herrera-Oropeza G, O'Brien-Gore C, Roy E, Wojtynska M, Spagnoli FM. 3D multicellular systems in disease modelling: From organoids to organ-on-chip. Front Cell Dev Biol. 2023 11:1083175. doi: 10.3389/fcell.2023.1083175. [PubMed]

2022

• Glorieux L, Sapala A, Willnow D, Moulis M, Edri S, Darrigrand JF, Schonblum A, Sakhneny L, Schaumann L, Gómez HF, Lang C, Conrad L, Guillemot F, Levenberg S, Landsman L, Iber D, Pierreux C, Spagnoli FM. Development of a 3D atlas of the embryonic pancreas for topological and quantitative analysis of heterologous cell interactions. Development. 2022 149(3):dev199655. doi: 10.1242/dev.199655. [PubMed]

• Brooks IR, Garrone CM, Kerins C, Kiar CS, Syntaka S, Xu JZ, Spagnoli FM, Watt FM. Functional genomics and the future of iPSCs in disease modeling. Stem Cell Reports. 2022 17(5):1033-1047. doi: 10.1016/j.stemcr.2022.03.019. [PubMed]

• Moulis M, Runser SVM, Glorieux L, Dauguet N, Vanderaa C, Gatto L, Tyteca D, Henriet P, Spagnoli FM, Iber D, Pierreux CE. Identification and implication of tissue-enriched ligands in epithelial-endothelial crosstalk during pancreas development. Sci Rep. 2022 12(1):12498. doi: 10.1038/s41598-022-16072-y. [PubMed]

2021

• Willnow D, Benary U, Margineanu A, Vignola ML, Konrath F, Pongrac IM, Karimaddini Z, Vigilante A, Wolf J, Spagnoli FM. Quantitative lineage analysis identifies a hepato-pancreato-biliary progenitor niche. Nature. 2021 Sep;597(7874):87-91. doi: 10.1038/s41586-021-03844-1. PMID: 34433966. [PubMed]

• Isaacson A, Spagnoli FM. Pancreatic cell fate specification: insights into developmental mechanisms and their application for lineage reprogramming. Curr Opin Genet Dev. 2021 Oct;70:32-39. doi: 10.1016/j.gde.2021.05.003. Epub 2021 May 29. PMID: 34062490. [PubMed]

• Oriola D, Spagnoli FM. Engineering life in synthetic systems. Development. 2021 Jul 15;148(14):dev199497. PMID: 34251450. [PubMed]

• Almeida N, Chung MWH, Drudi EM, Engquist EN, Hamrud E, Isaacson A, Tsang VSK, Watt FM, Spagnoli FM. Employing core regulatory circuits to define cell identity. EMBO J. 2021 May 17;40(10):e106785. doi: 10.15252/embj.2020106785. PMID: 33934382. [PubMed]

2020

• Cozzitorto C, Mueller L, Ruzittu S, Mah N, Willnow D, Darrigrand JF, Wilson H, Khosravinia D, Mahmoud AA, Risolino M, Selleri L, Spagnoli FM. A Specialized Niche in the Pancreatic Microenvironment Promotes Endocrine Differentiation. Dev Cell. 2020 Aug 20:S1534-5807(20)30627-4. doi: 10.1016/j.devcel.2020.08.003. Online ahead of print.

• Ruzittu S, Willnow D, Spagnoli FM. Direct Lineage Reprogramming: Harnessing Cell Plasticity between Liver and Pancreas. Cold Spring Harb Perspect Biol. 2020 Jul 1;12(7). pii: a035626. doi: 10.1101/cshperspect.a035626

Previous

• Helker CSM, Mullapudi S, Mueller LM, Kreuder F, Preussner J, Tunaru S, Skog O, Kwon H-B, Bonnavion R, Looso M, Offermanns S, Korsgren O, Spagnoli FM, Stainier DYR. (2019) A whole organism small molecule screen identifies novel regulators of pancreatic endocrine development. Development. 146(14). pii: dev172569. PMID: 31142539 [PubMed]

• Pancreas organogenesis: The interplay between surrounding microenvironment(s) and epithelium-intrinsic factors. Cozzitorto C, Spagnoli FM. Current Topics in Developmental Biology. 2019 [Link to publication]

• Robo signalling controls pancreatic progenitor identity by regulating Tead transcription factors. Escot S, Willnow D, Naumann H, Di Francescantonio S, Spagnoli FM. Nature Communications. 2018 9(1):5082. doi: 10.1038/s41467-018-07474-6. PMID: 30504829 [PubMed]

• Location matters for insulin-producing cells. Spagnoli FM. Nature. 2018 564(7734):50-51. doi: 10.1038/d41586-018-07490-y. Invited editorial. PMID: 30510228 [PubMed]

• The RhoGAP Stard13 controls insulin secretion through F-actin remodeling. Naumann, H., Rathjen, T., Poy, MN., Spagnoli, F.M. Molecular Metabolism. 2018 8:96-105. doi: 10.1016/j.molmet.2017.12.013. PMID: 29310936 [PubMed]

• Step-wise reprogramming of liver to pancreas progenitor state by the transcriptional regulator Tgif2. Cerdá-Esteban N, Naumann N, Ruzzittu S, Cozzitorto C, Pongrac IM, Hommel A, Mah N, Andrade-Navarro MA, Bonifacio E, Spagnoli FM. Nature Communications. 2017 8:14127. doi:10.1038/ncomms14127. PMID: 28193997 [PubMed]

• Setd7 is a regulator of pancreatic cell identity. Kofent J, Zhang J, Spagnoli FM. Development. 2016 143(19):3573-3581.

• Xenopus as a model system for studying pancreatic development and diabetes. Kofent J, Spagnoli FM. Semin Cell Dev Biol. 2016 Mar;51:106-16. Invited Review. PMID: 26806634 [PubMed]

• In vivo reprogramming for tissue repair: Recent breakthroughs and future challenges. Heinrich C, Spagnoli FM*, Berninger B*. Nature Cell Biology. 2015 17(3):204-11. Invited Review. *co-corresponding authors.

• Recessive mutations in PCBD1 gene cause a new type of early-onset diabetes. Simaite D, Kofent J*, Gong M, Ruschendorf F, Jia S, Arn P, Bentler K, Ellaway C,Kuehnen P, Hoffmann GK, Blau N, Spagnoli FM, Huebner N, Raile K. Diabetes. 2014 63:3557-64. *second author was a student in my laboratory. PMID: 24848070 [PubMed]

• Mutually exclusive signaling signatures define the hepatic and pancreatic progenitor cell lineage divergence. Rodríguez-Seguel E, Mah N, Naumann H, Pongrac IM, Cerdá-Esteban N, Fontaine JF, Wang Y, Chen W, Andrade-Navarro MA, Spagnoli FM. Genes & Development. 2013 27(17):1932-46. PMID: 24013505 [PubMed]

• Glimpse into Hox and Tale regulation of cell differentiation and reprogramming. Cerdá-Esteban N, Spagnoli FM. Dev. Dyn. 2014 243(1):76-87. Invited review. PMID: 24123411 [PubMed]

• Rho signalling restriction by the RhoGAP Stard13 integrates growth and morphogenesis in the pancreas. Petzold KM, Naumann H, Spagnoli FM. Development. 2013 140(1):126-35. PMID: 23175628 [PubMed]

• A system for ex vivo culturing of embryonic pancreas. Petzold KM, Spagnoli FM. J Vis Exp. 2012 Aug 27;(66):e3979. doi: 10.3791/3979. PMID: 22951988 [PubMed]

• The miR-430/427/302 family controls mesendodermal fate specification via species-specific target selection. Spagnoli FM*, Rosa A*, Brivanlou AH. Developmental Cell. 2009 Apr;16(4):517-27. *co-first authors (Faculty 1000). PMID: 19386261 [PubMed]

• The Gata5 target, TGIF2, defines the pancreatic region by modulating BMP signals within the endoderm. Spagnoli FM, Brivanlou AH. Development. 2008 Feb;135(3):451-61. PMID:  18094028 [PubMed]

• Balancing BMP signaling through integrated inputs into the Smad1 linker. Sapkota G, Alarcón C, Spagnoli FM, Brivanlou AH, Massagué J. Mol Cell. 2007 Feb 9;25(3):441-54. PMID: 17289590 [PubMed]